Radiance Q is a chemiluminescent HRP substrate with picogram sensitivity and a large linear dynamic range developed for CCD imaging. #Western blot sandwich plusRadiance Plus is a chemiluminescent HRP substrate with low-femtogram sensitivity for detection of low-abundance proteins.Radiance ECL is a chemiluminescent HRP substrate that provides long-lasting signal and picogram sensitivity.For fluorescent detection, the blot is imaged using a digital imager that has a light source to excite the fluorophore and has the correct filters to detect the emitted fluorescence. For chemiluminescent detection, the blot is incubated with a chemiluminescent substrate and the emitted light detected using film or a digital imager. AzureSpecta Labeling Kits contain sufficient reagents to label 100 mg of antibody quickly and easily with AzureSpecta fluorescent dyes, including spin columns to clean up the labeled antibody.įinally, the bound secondary antibodies are detected.Kits are also available with Fluorescent Blot Blocking Buffer. AzureSpectra Fluorescent Western Blotting Kits contain materials for 10 Western blots, including PVDF membranes, Washing Buffer, Blot Blocking Buffer, 2 Secondary Antibodies, and Background Quenching Sheets.HRP-conjugated Secondary Antibodies are available directed against primary antibodies from seven species.AzureSpectra Fluorescent Secondary Antibodies are available labeled with a selection of five fluorophores and directed against primary antibodies from seven species.Secondary antibodies are usually labeled with a fluorophore that can be detected directly, or bound to an enzyme like horseradish peroxidase (HRP) that reacts with a substrate to produce light (chemiluminescence) or a colored product that can be detected visually or on film. The presence of primary antibodies bound to their target protein on the blot is detected by binding a labeled secondary antibody to the primary antibody. Azure Transfer Buffer is formulated for enhanced protein transfer for improved sensitivity.Pre-cut Nitrocellulose Membranes are available in 10 cm x 15 cm and in two pore sizes.Pre-cut PVDF Membranes are available in three sizes, 9 cm x 7 cm, 10 cm x 15 cm, and 13 cm x 18 cm.Pre-cut Blotting Papers for transfer sandwiches are available in three sizes, 9 cm x 7 cm, 10 cm x 15 cm, and 13 cm x 18 cm.The Azure Aqua Power Supply can power up to four different modules simultaneously and can create and save protocols for electrophoresis and blotting.Cassettes and electrodes are color-coded so you can be sure you set up the transfer with the correct directionality. The Aqua Transfer Cell includes two cassettes, cooling units, and everything necessary for transfer.
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